Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 97, Issue 1, Pages 86-98Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/bit.21179
Keywords
intracellular pH; fast dynamics; yeast; benzoic acid; pulse
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pH affects many processes on cell metabolism, such as enzyme kinetics. To enhance the understanding of the living cells, it is therefore indispensable to have a method to monitor the pH in living cells. To accomplish this, a dynamic intracellular pH measurement method applying low concentration benzoic acid pulse was developed. The method was thoroughly validated and successfully implemented for measuring fast dynamic intracellular pH of Saccharomyces cerevisiae in response to a glucose pulse perturbation performed in the BioSCOPE set-up. Fast drop in intracellular pH followed by partial alkalinization was observed following the pulse. The low concentration benzoic acid pulse which was implemented in the method avoids the undesirable effects that may be introduced by benzoic acid to cell metabolism.
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