Journal
NEOPLASIA
Volume 9, Issue 5, Pages 418-426Publisher
NEOPLASIA PRESS
DOI: 10.1593/neo.07205
Keywords
senescence; cancer cell xenografts; immunodeficient mice; stroma; subrenal capsule
Categories
Funding
- NCI NIH HHS [P30 CA54174, P30 CA054174] Funding Source: Medline
- NIA NIH HHS [R37 AG012287, P01 AG020752, AG20752, AG12287] Funding Source: Medline
Ask authors/readers for more resources
Fibroblast cooperation with cancer cells in xenograft development was investigated by transplanting MDA-MB231 cells under the kidney capsule of immunodeficient mice. Control fibroblasts and fibroblasts subjected to stress-induced premature senescence by treatment with bleomycin were used. In other xenograft models, senescent fibroblasts have shown a growth- stimulatory effect greater than that of control cells. In this model, both types of fibroblasts accelerated the formation and growth of xenografts. Blood vessel development, as evidence by von Willebrand factor staining, was greatly accelerated by the presence of fibroblasts, and invasion into the kidney was also increased. Control and senescent fibroblasts had very similar effects. These actions of fibroblasts were partially recapitulated in in vitro experiments. Both control and senescent fibroblasts stimulated the tubulogenesis of endothelial cells in culture and stimulated the invasion of MDA-MB-231 cells through Matrigel in vitro. In this xenograft model, in which fibroblasts are cotransplanted with a cancer cell into an internal organ rather than subcutaneously, senescence was not an important factor in the effects of cotransplanted fibroblasts on growth, blood vessel development, and invasion. Therefore, cancer promotion by the senescence of adjacent stromal cells may be restricted to certain organ and tissue types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available