4.7 Article

Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa

Journal

JOURNAL OF CLINICAL MICROBIOLOGY
Volume 45, Issue 5, Pages 1618-1620

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01405-06

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Funding

  1. NIAID NIH HHS [P01 AI030731, AI-30731-13] Funding Source: Medline

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In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.

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