Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 5, Pages 677-684Publisher
SPRINGER
DOI: 10.1007/s10529-006-9297-y
Keywords
mammalian cells; non-viral gene delivery; recombinant protein; transient gene expression
Categories
Ask authors/readers for more resources
The expansion of the biologics pipeline depends on the identification of candidate proteins for clinical trials. Speed is one of the critical issues, and the rapid production of high quality, research-grade material for preclinical studies by transient gene expression (TGE) is addressing this factor in an impressive way: following DNA transfection, the production phase for TGE is usually 2-10 days. Recombinant proteins (r-proteins) produced by TGE can therefore enter the drug development and screening process in a very short time-weeks. With classical approaches to protein expression from mammalian cells, it takes months to establish a productive host cell line. This article summarizes efforts in industry and academia to use TGE to produce tens to hundreds of milligrams of r-proteins for either fundamental research or preclinical studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available