Journal
JOURNAL OF SEPARATION SCIENCE
Volume 30, Issue 7, Pages 1082-1087Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.200600394
Keywords
asymmetrical flow field-flow fractionation; exosome; FFF; miniaturized FFF channel; protein separation
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Asymmetrical flow field-flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size. By reducing the channel dimension, proteins or particulate materials can be separated within a few minutes without a significant loss in resolution. The mAFlFFF channel was applied for the separation of exosomes harvested from immortalized human mesenchymal stem cell line. It shows a potential to fractionate exosome vesicles according to sizes which can be useful for proteomic studies in relation to immunotherapeutic applications.
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