Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 850, Issue 1-2, Pages 168-176Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2006.11.023
Keywords
trimethylaminoethyl ester; fatty acids; stable isotope dilution; liquid chromatography/mass spectrometry; electrospray ionization; atherosclerosis
Funding
- NCI NIH HHS [R01CA91016] Funding Source: Medline
- NHLBI NIH HHS [P50HL70128] Funding Source: Medline
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The quantitative analysis of fatty acid composition in atherosclerotic plaques provides a way to monitor the underlying etiology of atherosclerosis. Previously, the method of choice for analyzing fatty acids in biological samples was gas chromatography/mass spectrometry (GC/MS); however, recent developments in electrospray ionization (ESI)/liquid chromatography (LC)/tandem mass spectrometry have made it a superior alternative. Previous research has largely focused on global analyses of intact lipids rather than more targeted analysis of the fatty acids themselves. We have now developed a targeted, stable isotope dilution LC-electrospray ionization/multiple reaction monitoring/MS method for the quantitative analysis of 10 fatty acids (myristic, palmitic, stearic, oleic, linoleic, alpha-linolenic, gamma-linolenic, arachidonic, eicosapentaenoic, and docosahexaenoic acids) using their trimethylaminoethyl ester (TMAE) derivatives to improve sensitivity. The method was validated, had a detection limit in the fmol range, and was used in the analysis of fatty acids in atherosclerotic plaques from carotid arteries. (c) 2006 Elsevier B.V. All rights reserved.
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