Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 73, Issue 10, Pages 3431-3436Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02702-06
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A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species.
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