Journal
ANDROLOGIA
Volume 47, Issue 6, Pages 685-693Publisher
WILEY
DOI: 10.1111/and.12319
Keywords
Cryopreservation; dimethylformamide; glycerol; Lama glama (llama); semen
Categories
Funding
- University of Buenos Aires (UBACyT) [20020100200051, 20020090200566]
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The aim of this study was to determine the effect of two equilibration temperatures (5 degrees C and room temperature) and two cryoprotectants (glycerol and dimethylformamide, both at 7%) on llama sperm cryopreservation. Llama ejaculates were divided into four aliquots. A lactose-EDTA-egg yolk (LEEY) extender with either 7% glycerol (LEEY-G) or 7% dimethylformamide (LEEY-DMF) was added to two of the aliquots, which were equilibrated for 20min at room temperature and subsequently frozen. The other two aliquots were extended in LEEY, cooled to 5 degrees C, then LEEY-G or LEEY-DMF was added, equilibrated for 20min at 5 degrees C and frozen. No significant differences (P>0.05) were observed in membrane function and chromatin condensation between any of the freeze-thawing protocols. Post-thaw motility was greater (P<0.05) in LEEY-DMF than LEEY-G. DNA fragmentation was not different between raw and frozen semen with LEEY-DMF but was high in all samples with glycerol. Our results indicate that 7% glycerol would be detrimental for llama spermatozoa, but further studies are needed to evaluate effectiveness if used at lower concentrations. Dimethylformamide preserved motility and DNA integrity of frozen-thawed llama spermatozoa and could be used to replace glycerol at the concentrations used in this study.
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