Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 5, Pages 731-735Publisher
SPRINGER
DOI: 10.1007/s10529-007-9312-y
Keywords
agarose; cellular adhesion; conjugate; encapsulation; gelatin
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Spherical capsules were prepared by extruding aqueous agarose-gelation conjugate solution into co-flowing liquid paraffin at 38 degrees C and cooling the resultant emulsion. Capsule diameter was controlled between 40 and 250 mu m by changing the velocity of the liquid paraffin. Adherent Crandall-Reese feline kidney cells enclosed in conjugate capsules of 141 +/- 23 mu m diam. had a higher degree of proliferation than those in unmodified agarose capsules. Mitochondrial activity, detected for cell-enclosing conjugate capsules normalized against unit volume of gel, was about double that of unmodified agarose capsules over 28 days. These results demonstrated the feasibility of agarose-gelatin conjugate as a material of cell-enclosing capsules.
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