Journal
ANDROLOGIA
Volume 46, Issue 1, Pages 42-49Publisher
WILEY
DOI: 10.1111/and.12040
Keywords
Cryopreservation; fatty acids; ovine; spermatozoa; testosterone
Categories
Funding
- Royan Institute, Tehran, Iran
- Saveh Branch of the Islamic Azad University, Saveh, Iran [PR 88.41103]
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The aim of this study was to investigate the effects of several dietary fatty acids (FAs) on semen quality and blood parameters in rams. We gave diet-supplemented treatments (35gday(-1)ram(-1)) by C16:0 (palm oil), C18:2 [sunflower oil (SO)] and an n-3 source [fish oil (FO)] to 12rams, who were fed for 15weeks during their breeding season. Semen was collected once per week. Semen samples were extended with Tris-based cryoprotective diluents, then cooled to 5 degrees C and stored in liquid nitrogen. Positive responses were seen with FO after 4weeks. The mean prefreezing semen characteristics improved with the intake of FO (P<0.05). Interestingly, maximum sperm output in FO was achieved 7.5x10(9) when compared to palm oil 5.3x10(9). Rams that received FO had the highest total testosterone concentrations (11.3ngml(-1) for FO, 10.8ngml(-1) for SO and 10.2ngml(-1) for palm oil) during the experiment (P<0.05). FO also improved the rams' sperm characteristics after thawing (P<0.05). Although C16:0 is a major saturated FA in ram sperm and all rams have been fed isoenergetic rations, the unique FAs of FO improved fresh semen quality and freezing ability compared to other oils.
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