Journal
HISTOCHEMISTRY AND CELL BIOLOGY
Volume 127, Issue 5, Pages 523-530Publisher
SPRINGER
DOI: 10.1007/s00418-007-0275-9
Keywords
subcutaneous connective tissue; alpha smooth muscle actin; fibroblast; nucleus; quantum dots
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Funding
- NCCIH NIH HHS [R01-AT01121] Funding Source: Medline
- NCRR NIH HHS [P20 RR16435] Funding Source: Medline
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Alpha smooth muscle actin (alpha-SMA) was recently shown to be present in mouse subcutaneous tissue fibroblasts in the absence of tissue injury. In this study, we used a combination of immunohistochemistry and correlative confocal scanning laser and electron microscopy to investigate the structural organization of alpha-SMA in relation to the nucleus. Furthermore, we explored colocalization analysis as a method for quantifying the amount of alpha-SMA in close approximation to the nucleic acid marker, 4',6-diamidino-2-phenyl-indole, dihydrochloride. Our findings indicate the presence of alpha-SMA within nuclear invaginations in close proximity to the nuclear membrane, but not in the nucleoplasm. Although the function of these alpha-SMA-rich nuclear invaginations is at present unknown, the morphology of these structures suggests their possible involvement in cellular and nuclear mechanotransduction as well as nuclear transport.
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