Journal
JOURNAL OF MOLECULAR MEDICINE-JMM
Volume 85, Issue 5, Pages 481-496Publisher
SPRINGER
DOI: 10.1007/s00109-006-0148-z
Keywords
adenovirus; fibromodulin; wound healing; dermal fibroblasts; scar formation; transforming growth factor-beta; matrix metalloproteinases; tissue-derived inhibitors of matrix metalloproteinase
Funding
- NCI NIH HHS [R01 CA98543, R01CA93796] Funding Source: Medline
- NIAMS NIH HHS [AR46031] Funding Source: Medline
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Fibromodulin, a member of the small leucine-rich proteoglycan family, has been recently suggested as a biologically significant mediator of fetal scarless repair. To assess the role of fibromodulin in the tissue remodeling, we constructed an adenoviral vector expressing human fibromodulin cDNA. We evaluated the effect of adenovirus-mediated overexpression of fibromodulin in vitro on transforming growth factors and metalloproteinases in fibroblasts and in vivo on full-thickness incisional wounds in a rabbit model. In vitro, we found that Ad-Fibromodulin induced a decrease of expression of TGF-beta(1) and TGF-beta(2) precursor proteins, but an increase in expression of TGF-beta(3) precursor protein and TGF-beta type II receptor. In addition, fibromodulin overexpression resulted in decreased MMP-1 and MMP-3 protein secretion but increased MMP-2, TIMP-1, and TIMP-2 secretion, whereas MMP-9 and MMP-13 were not influenced by fibromodulin overexpression. In vivo evaluation by histopathology and tensile strength demonstrated that Ad-Fibromodulin administration could ameliorate wound healing in incisional wounds. In conclusion, although the mechanism of scar formation in adult wounds remains incompletely understood, we found that fibromodulin overexpression improves wound healing in vivo, suggesting that fibromodulin may be a key mediator in reduced scarring.
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