Journal
BIOELECTROCHEMISTRY
Volume 70, Issue 2, Pages 356-362Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2006.05.003
Keywords
morin binding; deoxyribonucleate acid (DNA); glassy carbon electrode (GCE); chemical analytics
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A poly (tetrafluroethylene)-deoxyribonucleate acid (PTFE-DNA) film-modified glassy carbon electrode (GCE) has been fabricated. The electrochemical oxidation behaviors of morin as well as its interaction with DNA have been studied at PTFE-DNA film-modified GCE and bare GCE by electrochemical methods. This modified electrode shows an enhanced effectiveness towards the oxidation of morin. Importantly, as to the interaction between morin and DNA in solution, characteristic parameters such as the binding stoichiometry and association equilibrium constant according to the Hill model for cooperative binding have been determined on the basis of linear sweep voltammetry and chronocoulometry. (C) 2006 Elsevier B.V. All rights reserved.
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