Serotonin modulates the response of embryonic thalamocortical axons to netrin-1

Modifying serotonin ( 5- HT) abundance in the embryonic mouse brain disrupts the precision of sensory maps formed by thalamocortical axons ( TCAs), suggesting that 5- HT influences their growth. We investigated the mechanism by which 5- HT influences TCAs during development. 5- HT1B and 5- HT1D receptor expression in the fetal forebrain overlaps with that of the axon guidance receptors DCC and Unc5c. In coculture assays, axons originating from anterior and posterior halves of the embryonic day 14.5 dorsal thalamus responded differently to netrin- 1, reflecting the patterns of DCC and Unc5c expression. 5- HT converts the attraction exerted by netrin- 1 on posterior TCAs to repulsion. Pharmacological manipulation of 5- HT1B/1D receptors and intracellular cAMP showed the signaling cascade through which this modulation occurs. An in vivo correlate of altered TCA pathfinding was obtained by transient manipulation of 5- HT1B/1D receptor expression abundance in the dorsal thalamus by in utero electroporation. These data demonstrate that serotonergic signaling has a previously unrecognized role in the modulation of axonal responsiveness to a classic guidance cue.