Journal
MACROMOLECULAR BIOSCIENCE
Volume 7, Issue 5, Pages 579-588Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200600280
Keywords
bioengineering; biomaterials; flow perfusion seeding; mesenchymal stem cell; poly(L-lactic acid); RGD; tissue engineering
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Arg-Gly-Asp (RGD) has been widely utilized to increase cell adhesion to three-dimensional scaffolds for tissue engineering. However, cell seeding on these scaffolds has only been carried out statically, which yields low cell seeding efficiencies. We have characterized, for the first time, the seeding of rat mesenchymal stem cells on RGD-modified poly(L-lactic acid) (PLLA) foams using oscillatory flow perfusion. The incorporation of RGD on the PLLA foams improves scaffold cellularity in a dose-dependent manner under oscillatory flow perfusion seeding. When compared to static seeding oscillatory flow perfusion is the most efficient seeding technique. Cell detachment studies show that cell adhension is dependent on the applied flow rate, and that cell attachment is strengthened at higher levels of RGD modification.
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