Aging is associated with elevated intracellular calcium levels and altered calcium homeostatic mechanisms in hippocampal neurons

Aging is associated with increased vulnerability to neurodegenerative conditions such as Parkinson's and Alzheimer's disease and greater neuronal deficits after stroke and epilepsy. Emerging studies have implicated increased levels of intracellular calcium ([Ca2+](i)) for the neuronal loss associated with aging related disorders. Recent evidence demonstrates increased expression of voltage gated Ca2+ channel proteins and associated Ca2+ currents with aging. However, a direct comparison of [Ca2+](i) levels and Ca2+ homeostatic mechanisms in hippocampal neurons acutely isolated from young and mid-age adult animals has not been performed. In this study, Fura-2 was used to determine [Ca2+](i) levels in CA1 hippocampal neurons acutely isolated from young (4-5 months) and mid-age (12-16 months) Sprague-Dawley rats. Our data provide the first levels. Upon glutamate direct demonstration that mid-age neurons in comparison to young neurons manifest significant elevations in basal [Ca2+](i), stimulation and a subsequent [Ca2+](i) load, mid-age neurons took tonger to remove the excess [Ca2+](i) in comparison to young neurons, providing direct evidence that altered Ca2+ homeostasis may be present in animals at significantly younger ages than those that are commonly considered aged (>= 24 months). These alterations in Ca2+ dynamics may render aging neurons more vulnerable to neuronal death following stroke, seizures or head trauma. Elucidating the functionality of Ca2+ homeostatic mechanisms may offer an understanding of the increased neuronal loss that occurs with aging, and allow for the development of novel therapeutic agents targeted towards decreasing [Ca2+](i) levels thereby restoring the system's that maintain normal Ca2+ homeostasis in aged neurons. (c) 2007 Elsevier Ireland Ltd. All rights reserved.