Journal
LIVESTOCK SCIENCE
Volume 109, Issue 1-3, Pages 19-23Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.livsci.2007.01.027
Keywords
essential amino acids; catabolism; pigs; enterocytes; transamination
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This study was conducted to test the hypothesis that porcine jejunal mucosal cells can degrade all essential amino acids (EAA). Jejunal mucosal cells (primarily enterocytes) were isolated from 50-day-old healthy pigs and incubated at 37 degrees C for 45 min in Krebs buffer containing plasma concentrations of amino acids and one of the following L-[1-C-14]- or L-[U-C-14]-amino acids plus unlabeled tracers at 0.5, 2, or 5 mM: histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine. There was active transamination of leucine, isoleucine and valine in the cells (1.35-2.5 nmol/mg protein per 45 min at 2 mM), with most (67-71%) of their alpha-ketoacids released into the medium. In contrast, catabolism of methionine and phenylalanine was negligible (0.04-0.05 nmol/mg protein per 45 min at 2 mM) and that of other EAA was completely absent in these cells. These results indicate that intestinal mucosal cells are a site for the substantial degradation of branched-chain amino acids but not other EAA in pigs. (c) 2007 Elsevier B.V. All rights reserved.
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