Journal
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
Volume 263, Issue 1, Pages 71-81Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijms.2007.01.001
Keywords
negative ion mode electrospray ionization; disulfide bond; fourier transform mass spectrometry; electron detachment dissociation; infrared multiphoton dissociation
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Disulfide bonds generally show only limited cleavage in positive ion mode collision activated dissociation (CAD). However, it has been demonstrated that a reverse situation exists in negative ion mode in which preferential S-S and C-S bond cleavage occurs. Here, we show that electron detachment dissociation (EDD) and infrared multiphoton dissociation (IRMPD) of peptide anions containing disulfide linkages also result in preferential cleavage of S-S and C-S bonds. Resulting products are mainly radical ions in EDD whereas IRMPD produces even-electron product ions, as expected, thereby supporting different disulfide cleavage mechanisms for these two fragmentation processes. We also show that, in EDD, the presence of tryptophan can result in abundant side chain loss (129 Da), which effectively can compete with disulfide bond cleavage. (c) 2007 Elsevier B. V. All rights reserved.
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