Spontaneous IPSCs and glycine receptors with slow kinetics in wide-field amacrine cells in the mature rat retina

The functional properties of glycine receptors were analysed in different types of wide-field amacrine cells, narrowly stratifying cells considered to playa role in larger-scale integration across the retina. The patch-clamp technique was used to record spontaneous IPSCs (spIPSCs) and glycine-evoked patch responses from mature rat retinal slices (4-7 weeks postnatal). Glycinergic spIPSCs were blocked reversibly by strychnine (300 nm). Compared to previously described splPSCs in All amacrine cells, the splPSCs in wide-field amacrine cells displayed a very slow decay time course (tau(fast) similar to 15 ms; tau(slow) similar to 57 ms). The kinetic properties of spIPSCs in whole-cell recordings were paralleled by even slower deactivation kinetics of responses evoked by brief pulses of glycine (3 mm) to outside-out patches from wide-field amacrine cells (tau(fast) similar to 45 ms; tau(slow) similar to 350 ms). Non-stationary noise analysis of patch responses and spIPSCs yielded similar average single-channel conductances (similar to 31 and similar to 34 pS, respectively). Similar, as well as both lower- and higher-conductance levels could be identified from directly observed single-channel gating during the decay phase of spIPSCs and patch responses. These results suggest that the slow glycinergic spIPSCs in wide-field amacrine cells involve alpha 2 beta heteromeric receptors. Taken together with previous work, the kinetic properties of glycine receptors in different types of amacrine cells display a considerable range that is probably a direct consequence of differential expression of receptor subunits. Unique kinetic properties are likely to differentially shape the glycinergic input to different types of amacrine cells and thereby contribute to distinct integrative properties among these cells.