Journal
EXPERIMENTAL CELL RESEARCH
Volume 313, Issue 9, Pages 1943-1950Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2007.02.024
Keywords
hematopoetic stem cell; stem cell transplantation; lineage tracking; green fluorescent protein; immunohistochemistry; cell fate
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Funding
- Intramural NIH HHS [Z01 NS002996-04] Funding Source: Medline
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The green fluorescent protein (GFP) is among the most commonly used expression markers in biology. GFP-tagged cells have played a particularly important role in studies of cell lineage. Sensitive detection of GFP is crucially important for such studies to be successful, and problems with detection may account for discrepancies in the literature regarding the possible fate choices of stem cells. Here we describe a very sensitive technique for visualization of GFP. Using it we can detect about 90% of cells of donor origin while we could only see about 50% of these cells when we employ the methods that are in general use in other laboratories. In addition, we provide evidence that some cells permanently silence GFP expression. In the case of the progeny of bone marrow stem cells, it appears that the more distantly related they are to their precursors, the more likely it is that they will turn off the lineage marker. Published by Elsevier Inc.
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