Journal
CELL
Volume 129, Issue 4, Pages 773-785Publisher
CELL PRESS
DOI: 10.1016/j.cell.2007.03.037
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The immunological synapse (IS) is a junction between the T cell and antigen-presenting cell and is composed of supramolecular activation clusters (SMACs). No studies have been published on naive T cell IS dynamics. Here, we find that IS formation during antigen recognition comprises cycles of stable IS formation and autonomous naive T cell migration. The migration phase is driven by PKC theta, which is localized to the F-actin-dependent peripheral (p)SMAC. PKC theta(-/-) T cells formed hyperstable, IS in vitro and in vivo and, like WT cells, displayed fast oscillations in the distal SMAC, but they showed reduced slow oscillations in pSMAC integrity. IS reformation is driven by the Wiscoft Aldrich Syndrome protein (WASp). WASp(-/-) T cells displayed normal IS formation but were unable to reform IS after migration unless PKC theta was inhibited. Thus, opposing effects of PKC theta and WASp control IS stability through pSMAC symmetry breaking and reformation.
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