Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 104, Issue 21, Pages 8930-8934Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0701157104
Keywords
coenzyme F-420; microarrays; Methanococcus maripaludis
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Funding
- NIGMS NIH HHS [R01 GM060403, GM60403, GM74783, R24 GM074783] Funding Source: Medline
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The use of molecular hydrogen as electron donor for energy generation is a defining characteristic of the hydrogenotrophic methanogens, an ancient group that dominates the phylum Euryarchaeota. We present here a global study of changes in mRNA abundance in response to hydrogen availability for a hydrogenotrophic methanogen. Cells of Methanococcus maripaludiswere grown by using continuous culture to deconvolute the effects of hydrogen limitation and growth rate, and microarray analyses were conducted. Hydrogen limitation markedly increased mRNA levels for genes encoding enzymes of the methanogenic pathway that reduce or oxidize the electron-carrying deazaflavin, coenzyme F-420. F-420-dependent redox functions in energy-generating metabolism are characteristic of the methanogenic Archaea, and the results show that their regulation is distinct from other redox processes in the cell. Rapid growth increased mRNA levels of the gene for an unusual hydrogenase, the hydrogen -de pendent methylenetetrahydromethanopterin dehydrogenase.
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