Journal
BIOCHEMISTRY
Volume 46, Issue 20, Pages 6118-6125Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bi7000032
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Funding
- Ministry of Education, Science & Technology (MoST), Republic of Korea [kaist66] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- NIA NIH HHS [1R21AG024114-01A2] Funding Source: Medline
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The abnormal deposition and aggregation of beta-amyloid (A beta) on brain tissues are considered to be one of the characteristic neuropathological features of Alzheimer's disease (AD). Environmental conditions such as metal ions, pH, and cell membranes are associated with A beta deposition and plaque formation. According to the amyloid cascade hypothesis of AD, the deposition of A beta 42 oligomers as diffuse plaques in vivo is an important earliest event, leading to the formation of fibrillar amyloid plaques by the further accumulation of soluble A beta under certain environmental conditions. In order to characterize the effect of metal ions on amyloid deposition and plaque growth on a solid surface, we prepared a synthetic template by immobilizing A beta oligomers onto a N-hydroxysuccinimide ester-activated solid surface. According to our study using ex situ atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FT-IR), and thioflavin T (ThT) fluorescence spectroscopy, Cu2+ and Zn2+ ions accelerated both A beta 40 and A beta 42 deposition but resulted only in the formation of amorphous aggregates. In contrast, Fe3+ induced the deposition of fibrillar amyloid plaques at neutral pH. Under mildly acidic environments, the formation of fibrillar amyloid plaques was not induced by any metal ion tested in this work. Using secondary ion mass spectroscopy (SIMS) analysis, we found that binding Cu ions to A beta deposits on a solid template occurred by the possible reduction of Cu ions during the interaction of A beta with Cu2+. Our results may provide insights into the role of metal ions on the formation of fibrillar or amorphous amyloid plaques in AD.
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