Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1150, Issue 1-2, Pages 85-92Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.09.008
Keywords
comprehensive two-dimensional liquid chromatography; time-of-flight mass spectrometry; Stevia rebaudiana
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Comprehensive two-dimensional liquid chromatography (LC x LC) connected on-line to electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS) was employed for analysis of aqueous extract of Stevia rebaudiana. Different combinations of strong cation-exchange (SCX), amino (NH,), and octadecyl siloxane (C-18) stationary phases were tested in the separation of all nine known sweet Stevia glycosides. A combination of Cis as the first-dimension column and NH2 as the second-dimension column fully separated all the glycosides from the matrix. The method proved to be quantitative and repeatable. The limit of detection (S/N = 3) for stevioside, a widely used natural sweetener, was 43.4 ng/g in dry leaves. The RSD for retention times was <0.1% and that of peak areas 4.5%. (C) 2006 Elsevier B.V. All rights reserved.
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