Journal
ANATOMICAL SCIENCE INTERNATIONAL
Volume 90, Issue 4, Pages 229-234Publisher
SPRINGER
DOI: 10.1007/s12565-014-0252-z
Keywords
Fasciculus retroflexus; Interpeduncular; nucleus; Medial habenular nucleus; Topographic map; Transgenic mice
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [13210021, 21220009]
- University of Tsukuba in Tsukuba, Japan
- Grants-in-Aid for Scientific Research [13210021, 21220009] Funding Source: KAKEN
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We generated transgenic mice lines with a construct consisting of the zif268/egr1 promoter and the gene for the normal long-life yellow fluorescent protein (Venus) with a membrane localization sequence. One of the lines exhibited topographic labeling in the medial habenular nucleus (MHb) during postnatal development, which confirmed the previous findings that the medial, lateral, and dorsal areas of MHb project to the ventral, dorsal, and lateral parts of the interpeduncular nucleus, respectively. In addition, the membranous localization of the labeling allowed us to observe spacial arrangement of the labeled axons in the fasciculus retroflexus (FR) in the transgenic mice. Here, we report topographic sorting of the MHb axons in the FR. At postnatal day (P) 5 and P10, the labeled axons from the medial MHb were fasciculated and ran through the narrow path in the core of the FR. At P24, the labeled axons from the medial and dorsal MHb were fasciculated and ran through the broad path in the FR core. No labeling occurred in the lateral MHb throughout development; correspondingly, parts of the FR core remained unlabeled. The results indicated that the axons from the medial and dorsal areas of the MHb are grouped together in the FR of this transgenic line and are sorted out from the axons from the lateral MHb.
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