Multiplicity editing including quaternary carbons:: improved performance for the 13C-DEPTQ pulse sequence

An improved version of the DEPTQ experiment yielding the signal and multiplicity information for all carbon types including the signals of quaternary carbons is proposed. It encompasses all the known advantages of the basic DEPT experiment. In comparison to the original version, signals of the sensitivity-limiting quaternary carbons are markedly increased: the initial C-13 pulse may be adjusted to the Ernst angle, the NOE build-up period is prolonged by the split relaxation delay and a partial recovery of signal losses due to instrumental imperfections is achieved by the incorporation of composite adiabatic C-13 refocussing pulses. Furthermore, pure absorption lineshapes for all carbon types are obtained with only one single scan. These attributes make this experiment attractive for C-13 analysis of small molecules (including spectral editing), particularly in high-throughput analysis laboratories. Copyright (C) 2007 John Wiley & Sons, Ltd.