Journal
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
Volume 296, Issue 1, Pages 31-39Publisher
WILEY
DOI: 10.1002/ar.22612
Keywords
oxidative stress; autophagy; apoptosis; ubiquitinated proteins; endoplasmic reticulum stress
Categories
Funding
- National Natural Science Foundation of China [81141099]
- Natural Science Foundation of Jilin Provincial Science and Technology Department [201215103]
- Jilin Provincial Education Department [2012-394, 2011-117]
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The role of lysosomal system in oxidative stress-induced apoptosis in cancer cells is not fully understood. Menadione is frequently used as oxidative stress model. It is indicated that menadione could induce autophagy in Hela cells. In the present study, we examined whether the lysosomal inhibitor, ammonium chloride (NH4Cl) could prevent the autophagy flux by inhibiting the fusion of autophagosomes with lysosomes and enhance apoptosis induced by menadione via mitochondrial pathway. The results demonstrated generation and accumulation of reactive oxygen species and increased levels of ubiquitinated proteins and GRP78 in cells treated with both menadione and NH4Cl. Our data indicates that lysosomal system through autophagy plays an important role in preventing menadione-induced apoptosis in Hela cells by clearing misfolded proteins, which alleviates endoplasmic reticulum stress. Anat Rec, 296: 31-39, 2013. (C) 2012 Wiley Periodicals, Inc.
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