Journal
BIOTECHNOLOGY LETTERS
Volume 29, Issue 6, Pages 871-875Publisher
SPRINGER
DOI: 10.1007/s10529-007-9323-8
Keywords
Escherichia coli; Hendra virus; Nipah virus; phosphoprotein; serological differentiation; viral infections
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The genus Henipavirus in the family Paramyxoviridae compromises two newly identified dangerous pathogens, Nipah virus and Hendra virus. Phosphoprotein of the two viruses is one of the major immunodominant antigens and the most divergent protein in the viral genomes. We have now expressed two pairs of truncated phosphoproteins of the two viruses in Escherichia coli in a soluble form using a vector tailored from pET32a. The truncated recombinant phosphoproteins were purified with His-Tag affinity chromatography and their antigenicity was determined by western blotting and ELISA. The longer pair of truncated recombinant phosphoproteins, covering amino acid residues 4-550, was more antigenic than the shorter one and of potential utility in the serological differentiation of henipavirus infections.
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