Journal
MOLECULAR AND CELLULAR NEUROSCIENCE
Volume 35, Issue 2, Pages 249-260Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mcn.2007.02.020
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- Wellcome Trust [074941, 077100] Funding Source: Medline
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A preconditioning sciatic nerve crush promotes the capacity of adult sensory neurons to regenerate following a subsequent injury to their axons. The increase in regeneration is detected in cultures of dissociated neurons, as an earlier and enhanced rate of neurite elongation. We compare neurotrophin-stimulated neurite outgrowth from sensory neurons on laminin and fibronectin. There is a poor response of sensory neurons to fibronectin in comparison to laminin, but this is enhanced by a preconditioning lesion to the sciatic nerve 7 days prior to culture. By using specific integrin-binding fibronectin fragments and function-blocking antibodies, we demonstrate that the enhanced preconditioned neurite outgrowth on fibronectin is largely mediated by alpha 5 beta 1 integrin. Preconditioning injury alter the subcellular localisation of alpha 5 integrin in preconditioned neurites. We show that alpha 5 integrin locatises to adhesion complexes in the growth cone and neurites of preconditioned neurons, but not control neurons. (c) 2007 Elsevier Inc. All rights reserved.
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