4.0 Article

Generation of a germ cell-specific mouse transgenic Cre line, Vasa-Cre

Journal

GENESIS
Volume 45, Issue 6, Pages 413-417

Publisher

WILEY
DOI: 10.1002/dvg.20310

Keywords

Cre; Vasa; germ cells; gonad

Funding

  1. NICHD NIH HHS [R01 HD048690-02, R01 HD048690-03, R01-HD04 8690, R01 HD048690] Funding Source: Medline

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Cell type-specific genetic modification using the Cre/IoxP system is a powerful tool for genetic analysis of distinct cell lineages. Because of the exquisite specificity of Vasa expression (confined to the germ cell lineage in invertebrate and vertebrate species), we hypothesized that a Vasa promoter-driven transgenic Cre line would prove useful for the germ cell lineage-specific inactivation of genes. Here we describe a transgenic mouse line, Vasa-Cre, where Cre is efficiently and specifically expressed in germ cells. Northern analysis showed that transgene expression was confined to the gonads. Cre-mediated recombination with the Rosa26-lacZ reporter was observed beginning at similar to e15, and was > 95% efficient in male and female germ cells by birth. Although there was a potent maternal effect with some animals showing more widespread recombination, there was no ectopic activity in most adults. This Vasa-Cre transgenic line should thus prove useful for genetic analysis of diverse aspects of gametogenesis and as a general deletor line.

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