Journal
ANALYTICAL SCIENCES
Volume 30, Issue 9, Pages 897-902Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.30.897
Keywords
Carbon nanosphere; sodium alginate; hybrid material; amperometric biosensor
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Funding
- National Natural Science Foundation of China [21205050, 21205051]
- China Postdoctoral Science Foundation [2012M511224]
- Natural Science Foundation of Jiangsu University [12JDG038]
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In this study, we developed a highly sensitive amperometric biosensor for glucose detection based on glucose oxidase immobilized in a novel carbon nanosphere (CNS)/sodium alginate (SA) composite matrix. This hybrid material combined the advantages of CNS and natural biopolymer SA. This composite film was characterized by scanning electron microscope, electrochemical impedance spectroscopy and UV-vis, which indicated that the hybrid material was suitable for immobilization of glucose oxidase. Various experimental conditions were investigated that influenced the performance of the biosensor, such as pH, applied potential and temperature. Under the optimum conditions, the biosensor showed excellent performance for glucose over a wide linear concentration range from 1.0 x 10(-6) to 4.6 x 10(-3) M with a detection limit of 0.5 mu M based on a signal-to-noise ratio of 3. Furthermore, the biosensor exhibited excellent long-term stability and satisfactory reproducibility.
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