Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 16, Issue 5, Pages 9804-9830Publisher
MDPI AG
DOI: 10.3390/ijms16059804
Keywords
microfluidics; single-cell electrical property analysis; impedance flow cytometry; high throughput
Funding
- National Natural Science Foundation of China [61201077, 81261120561, 61431019]
- National Basic Research Program of China (973 Program) [2014CB744600]
- National High Technology Research and Development Program of China (863 Program) [2014AA093408]
- Chang Gung Memorial Hospital in Taiwan [CMRPD2C0102, CMRPD2D0041]
- Beijing NOVA Program of Science and Technology
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This article reviews recent developments in microfluidic impedance flow cytometry for high-throughput electrical property characterization of single cells. Four major perspectives of microfluidic impedance flow cytometry for single-cell characterization are included in this review: (1) early developments of microfluidic impedance flow cytometry for single-cell electrical property characterization; (2) microfluidic impedance flow cytometry with enhanced sensitivity; (3) microfluidic impedance and optical flow cytometry for single-cell analysis and (4) integrated point of care system based on microfluidic impedance flow cytometry. We examine the advantages and limitations of each technique and discuss future research opportunities from the perspectives of both technical innovation and clinical applications.
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