Journal
NATURE METHODS
Volume 4, Issue 6, Pages 491-493Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/nmeth1051
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- NIGMS NIH HHS [U54 GM074958] Funding Source: Medline
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Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mu g (6 mu l, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.
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