Journal
ANALYTICAL SCIENCES
Volume 27, Issue 5, Pages 483-488Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.27.483
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Funding
- Fundacao para a Ciencia e Tecnologia (FCT)
- Fundo Social Europeu (FSE) [SFRH/BD/29857/2006]
- MCTES (Ministerio da Ciencia, Tecnologia e Ensino Superior)
- Fundação para a Ciência e a Tecnologia [SFRH/BD/29857/2006] Funding Source: FCT
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High-throughput cupric ion reducing antioxidant capacity (CUPRAC) methods were developed for assessment of total antioxidant capacity (TAC) in urine and serum, based on reduction of Cu(II)-neocuproine complex to highly colored Cu(I)-neocuproine complex, measured spectrophotometrically at 450 nm. The reaction time was significantly reduced from 30 to 4 min by application of a calibration compound (uric acid) with kinetic behavior similar to that shown by urine samples. The method was implemented in a rnicroformat (96 well plates) and also in an automatic fashion (flow injection analysis, FIA). A determination throughput value of 288 h(-1) (microplate method) or of 15 h(-1) (automatic FIA) was attained. Application of both methods to human serum (SRM 909b, level I) and urines (n = 9) provided TAC values in agreement with those of the end-point batch method.
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