Journal
ANALYTICAL SCIENCES
Volume 27, Issue 10, Pages 999-1005Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.27.999
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Funding
- Japan Society for the Promotion of Science [21590745]
- Grants-in-Aid for Scientific Research [21590745] Funding Source: KAKEN
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A rapid, specific, and sensitive method for the simultaneous quantitation of organophosphates (fenitrothion (MEP), malathion, and phenthoate (PAP)), glufosinate (GLUF), and glyphosate (GLYP) in human serum and urine by gas chromatography-mass spectrometry (GC-MS) has been validated. All of the targeted compounds together with the internal standard were extracted from the serum and urine using a mix-mode TiO-C-18 monolithic spin column. The recovery of organophosphates from serum and urine ranged from 12.7 to 49.5%. The recovery of GLUF and GLYP from serum and urine ranged from 1.9 to 7.9%. The intra- and inter-accuracy and precision (expressed as relative standard deviation, %RSD) were within 96.7 - 107.7% and 4.0 - 13.8%, respectively. The detection and quantitation limits for serum and urine were 0.1 and 0.1 mu g/ml, respectively, for organophosphates, 0.1 and 0.5 mu g/ml, respectively for GLUF and GLYP. The method had linear calibration curves ranging from 0.1 to 25.0 mu g/ml for organophosphates and 0.5 - 100.0 mu g/ml for GLUF, and GLYP. The validated method was successfully applied to a clinical GLYP poisoning case.
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