Journal
ANALYTICAL SCIENCES
Volume 27, Issue 5, Pages 465-469Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.27.465
Keywords
-
Categories
Funding
- New Energy and Industrial Technology Development Organization (NEDO) Japan
- Ministry of Education, Culture, Sports, Science and Technology, Japan [20106008]
- Grants-in-Aid for Scientific Research [09J03300, 20106008] Funding Source: KAKEN
Ask authors/readers for more resources
An on-chip enzyme-linked immunosorbent assay combined with an electrochemical detection method (EC-ELISA) was employed to detect a leptin, one of the most important adipose derived hormones, using gold electrodes modified with a tri(ethylene glycol) terminated short alkanethiol (TEGCnSH, Cn = (CH2)n, n = 2, 4, 6, and 8) monolayer. These TEGCnSH monolayers on gold electrodes can suppress non-specific protein adsorption without affecting the electrochemical activity required for detecting p-aminophenol (PAP), which is an alkaline phosphatase (ALP) product. We measured leptin with a highly sensitive detection range (100 pg mL(-1) to 10 ng mL(-1) level) and with the desired detection limit (13.6 pg mL(-1)) by using electrochemical detection. For detecting leptin, the EC-ELISA method using TEGC4SH modified gold electrode with a poly(dimethylsiloxane) based microchannel was superior to the conventional ELISA method. With the EC-ELISA method, we were able to measure leptin with a satisfactory detection range and a pg level detection limit within 30 min, which is a much lower detection level than that obtained with conventional plate based ELISA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available