Journal
ANALYTICAL SCIENCES
Volume 26, Issue 1, Pages 75-81Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.26.75
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)
Ask authors/readers for more resources
Serotonin-bonded silica was developed for specific extraction of sialic-acid-containing glycans and glycopeptides. Serotonin, coupled by reductive amination with aldehyde silica particles via its ethylamino group, has strong affinity to sialic acid in glycan chain termini. Sialylated glycans trapped on serotonin silica particles are released by washing with ammonium acetate solution, providing highly efficient specific trap and release glycoconjugates. With >100 mu mol/g adsorption capacity, the particles are applicable to purify labeled glycans after derivatization with 2-aminopyridine to remove excess reagents. Serotonin silica efficiently enriches sialic-acid-containing glycopeptides from tryptic digests for LC/MS analysis of glycans' heterogeneity in glycoproteins.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available