Journal
ANALYTICAL SCIENCES
Volume 25, Issue 3, Pages 339-346Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.339
Keywords
-
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [17655059, 19350045, 20043011, 20050007]
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
Ask authors/readers for more resources
In this paper, we describe systematic detailed considerations of the feasibility of using various metal nanoparticles for organic-matrix-free surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). In order to avoid the influence of organic molecules on the nanoparticles, stabilizer-free bare nanoparticles of Ag, Au, Cu and Pt were prepared by laser ablation. Although all metal nanoparticles absorbed N-2 laser light (337 nm) energy, the performance of desorption/ionization of a representative peptide, angiotensin 1, strongly depended on the metal element. Citrate buffer was used as a proton source; it reduced the amount of alkali cation adducts present. Then, protonated molecules of analytes predominated in the mass spectra when Au and Pt nanoparticles were used. Pt nanoparticles showed the highest performance in SALDI-MS, owing to their smaller heat conductivity and higher melting temperature. The selective desorption of a cationic surfactant with longer alkyl chains and a peptide with methionine was also observed.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available