Journal
ANALYTICAL SCIENCES
Volume 25, Issue 6, Pages 779-784Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.779
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Funding
- Ministry of Science, Technology and Innovation, Malaysia [UKM-MGI-NBD0021-2007]
- OUP Research Grant 2009
- Universiti Kebangsaan Malaysia
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Electrochemical biosensors for phenolic compound determination were developed by immobilization of tyrosinase enzyme in a series of methacrylic-acrylic based biosensor membranes deposited directly using a photocuring method. By modifying the hydrophilicity of the membranes using different proportions of 2-hydroxyethyl methacrylate (HEMA) and butyl acrylate (nBA), we developed biosensor membranes of different hydrophilic characters. The differences in hydrophilicity of these membranes led to changes in the sensitivity of the biosensors towards different phenolic compounds. In general biosensors constructed from the methacrylic-acrylic based membranes showed the poorest response to catechol relative to other phenolic compounds, which is in contrast to many other biosensors based on tyrosinase. The decrease in hydrophilicity of the membrane also allowed better selectivity towards chlorophenols. However, phenol biosensors constructed from the more hydrophilic membrane materials demonstrated better analytical performance towards phenol compared with those made from less hydrophilic ones. For the detection of phenols, these biosensors with different membranes gave detection limits of 0.13 - 0.25 mu M and linear response range from 6.2 - 54.2 mu M phenol. The phenol biosensors also showed good phenol recovery from landfill leachate samples (82 - 117%).
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