Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1151, Issue 1-2, Pages 108-114Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.chroma.2006.11.078
Keywords
mixer-settler counter-current chromatography; barricaded spiral disk; assembly; coil planet centrifuge; purification of biopolymers
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A novel spiral disk is designed by placing barricades at 6 mm intervals in the middle of the spiral channel to divide the channel into multiple sections. Glass beads are placed in every other section so that the planetary motion produces repetitive mixing and settling of polymer phase systems. Performance of this mixer-settler spiral disk assembly was examined for separation of lysozyme and myoglobin with a polymer phase system. The best results were obtained with a spiral disk equipped with barricades with openings ranging from 1.2 to 0.4 mm on each side at a high revolution speed up to 1200 rpm. (C) 2006 Elsevier B.V. All rights reserved.
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