Journal
ANALYTICAL SCIENCES
Volume 25, Issue 11, Pages 1327-1331Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.25.1327
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Funding
- Hunan Provincial Education Department [06B022]
- China Postdoctoral Science Foundation [20070420797]
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A novel nanoparticles-based fluorescence detection method has been developed by taking advantage of magnetic separation and amplified fluorescence detection. This DNA sensor relies on a sandwich hybridization strategy, in which the DNA targets are first hybridized to captured oligonucleotide probes immobilized on magnetic nanoparticles, and then hybridized with thiol-modified oligonucleotide probes immobilized on gold nanoparticles. Subsequently, the amplified DNA signals are detected in the form of bio-bar-code DNA using a chip-based fluorescence detection method. The result showed that the detection limit of target DNA probes is 1 pM. Complementary and mismatched sequences were clearly distinguished, and the ratio of the background-subtracted fluorescence values for complementary and single-base mismatched oligonucleotide was 2.12:1. This new system can be applied to both DNA detection and immunoassay, and has broad potential applications in disease diagnosis and immunoassay.
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