Journal
CRYOBIOLOGY
Volume 54, Issue 3, Pages 305-309Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2007.03.003
Keywords
expanded blastocyst; vitrification; cut standard straw; microbial contamination; aseptic method
Categories
Ask authors/readers for more resources
Vitrification of laser treated human blastocysts using reduced concentrations of permeable cryoprotectants was carried out by submerging cut standard straws (CSS) into liquid nitrogen. The CSS were made by cutting a standard 0.25 ml straw at an angle of similar to 45 degrees. After laser assisted hatching, 6 day blastocysts (n = 250) were loaded into droplets of similar to 0.75 mu l in the CSS and were either plunged directly into liquid nitrogen or first encased in a standard 0.5 ml straw (aseptic technique) before being vitrified. Permeable cryopmtectants (ethylene glycol + Me2SO) at concentrations of 15% and 20% v:v were tested for their effect on post warming re-expansion and post transfer pregnancy rates. Our results indicate that the use of reduced concentrations of cryoprotectants and aseptic packaging of blastocysts did not have any statistically significant impact on the study outcomes. (c) 2007 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available