4.6 Review

Advances in loop-mediated isothermal amplification: integrated with several point-of-care diagnostic methods

Journal

ANALYTICAL METHODS
Volume 6, Issue 19, Pages 7585-7589

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ay00330f

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Funding

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology
  2. Research Center for Eco-Environmental Sciences
  3. Chinese Academy of Sciences [KF201205]
  4. Natural Science Foundation of Shaanxi Province [2012JQ2011]
  5. Natural Science Foundation from Educational Department, Shaanxi Province [12JK0623]

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Recent outbreaks linked to pathogenic bacteria heighten the need to develop rapid, sensitive, portable and low-cost pathogen diagnostic systems. Loop-mediated isothermal amplification (LAMP) is a powerful and novel gene amplification method emerging as a simple rapid diagnostic tool for early detection and identification of microbial disease. Herein, we highlight the recent advances concerning LAMP which is integrated with several innovative detection technologies, thus, this article also reviews the extensive application of these basic techniques in food-borne bacterial detection.

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