Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 103, Issue 6, Pages 568-571Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.103.568
Keywords
metal-reducing bacteria; Shewanella algae; reductive deposition; nanoparticles; gold; X-ray; absorption near-edge structure spectroscopy (XANES)
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X-ray absorption near-edge structure spectroscopy (XANES) was successfully employed to determine the gold valence in the metal-reducing bacterium Shewanella algae after exposure to a 1 mM aqueous HAuCl(4) solution for 10-120 min. XANES spectra revealed the oxidation state of gold in the bacterial cells to be Au(0) without any contribution from Au(Ill), demonstrating that S. algae cells can reduce AuCl(4)(-) ions to elemental gold. Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis confirmed that gold nanoparticles 5-15 nm in size were deposited in the periplasmic space of the bacterial cells; a preferable, cell surface location for the easy recovery of biogenic nanoparticles.
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