Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 852, Issue 1-2, Pages 229-234Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jchromb.2007.01.022
Keywords
isothiocyantes; biomarker; synthesis; 1,3-benzodithiole-2-thione; N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine
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A well-known method for quantification of isothiocyanates (ITCs) and their metabolites is the condensation reaction with 1,2-benzenedithiole to produce 1,3-benzodithiole-2-thione, which can be quantified by high-performance liquid chromatography. Standards of an ITC metabolite and 1,3-benzodithiole-2-thione are required for this assay but are not commercially available. In the present study, we report on an improved synthesis of the ITC metabolite N-acetyl-S-(N-4-methylsulfinylbutylthiocarbamoyl)-L-cysteine and 1,3-benzodithiole-2-thione. The standards were used to quantify the urinary excretion of ITCs from 10 healthy subjects who consumed 350 g broccoli. The excretion was investigated throughout 48 h showing a cumulative urinary ITC excretion of 49.1 +/- 25.2% of the dose. (C) 2007 Elsevier B.V. All rights reserved.
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