Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 104, Issue 2, Pages 191-194Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.SC0070119
Keywords
membrane; interface tissue culture; organotypic culture; hippocampal slice
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Low-cost, simple procedures for organotypic tissue cultures are desirable for high-throughput biological experiments such as large-scale medical/drug screening. We present a practical and economical method to cultivate brain slices using hydrophilic filtration membranes. With a cost reduction of more than 90%, this technique allows us to prepare hippocampal slice cultures that are morphologically and functionally indistinguishable from those obtained by the widely used Millicell-CM (R) method.
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