Simultaneous determination of amantadine, rimantadine and chlorpheniramine in animal-derived food by liquid chromatography-tandem mass spectrometry after fast sample preparation

A fast method for simultaneous determination of amantadine, rimantadine, and chlorpheniramine in seven animal derived samples including pork, chicken, duck, pig liver, chicken liver, pig kidney, and egg was developed using liquid chromatography-tandem mass spectrometry, and employed a new multifunctional syringe filter that makes the cleanup procedure simple and rapid based on the QuEChERS (quick, easy, cheap, effective, rugged and safe) method. The method was validated using amantadine-d(15), rimantadine-d(4) and chlorpheniramine-d(6) as internal standards for three analytes. Good linearities (R-2 > 0.9938) were obtained over the concentration range from 2 mu g L-1 to 200 mu g L-1 for amantadine and rimantadine, and from 0.2 mu g L-1 to 20 mu g L-1 for chlorpheniramine. The precision was evaluated by intra- and inter-day assays and the relative standard deviations were all within 9.85%. Mean recoveries ranged from 89.9% to 105%. The limits of detection and quantification were 0.5 and 1.0 mu g kg(-1) for both amantadine and rimantadine, and 0.05 and 0.1 mu g kg(-1) for chlorpheniramine, respectively. The application of the developed method in real samples showed that amantadine and chlorpheniramine were respectively detected with percentages of 3.7% and 0.3% in all tested samples.