Journal
PROTEOMICS
Volume 7, Issue 11, Pages 1746-1752Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/pmic.200600782
Keywords
multiplexing; peptide isoelectric focusing; relative quantitation
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Immobilized pH gradient isoelectric focusing (IPG-IEF) has. emerged as a highly promising alternative to strong-cation exchange fractionation as the first dimension in shot-gun proteomics. Herein is shown the compatibility of this method with iTRAQ isotope labeling for relative quantitation and validation of sequence matches from database searching.
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