Dyadobacter koreensis sp nov., isolated from fresh water

A non-motile, rod-shaped, light-yellow-pigmented bacterium, designated strain WPCB159(T), was isolated from freshwater samples collected from the Woopo wetland in Korea. The cells were Gram-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were C-16:1 omega 7c (34.8%), iso-C-15:0 (24.2%) and C-16:0 (9.4%). The DNA G + C content was 44 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB159(T) forms a lineage within the genus Dyadobacter (family 'Flexibacteraceae') and is closely related to Dyadobacter hamtensis HHS 11(T) (97.8% sequence similarity) and to other members of the genus Dyadobacter (95.2-96.8% sequence similarity). The phenotypic characteristics and DNA-DNA hybridization relatedness data indicate that strain WPCB159(T) should be distinguished from D. hamtensis HHS 11(T). On the basis of the evidence presented in this study, strain WPCB159(T) represents a novel species of the genus Dyadobacter, for which the name Dyadobacter koreensis sp. nov. is proposed. The type strain is WPCB159(T) (=KCTC 12537(T) = NBRC 101116(T)).