Journal
ANALYTICAL METHODS
Volume 5, Issue 16, Pages 3927-3932Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3ay40099a
Keywords
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Funding
- National Basic Research Program of China [2013CB934304]
- National Natural Science Foundation of China [11175204]
- One-Hundred-Talent Program of the Chinese Academy of Sciences
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A label-free approach to the detection of coralyne using aptamer modified silica-Au/core-shell nanoparticles (denoted as SiO2@Au core-shell nanoparticles) through surface enhanced Raman scattering (SERS) spectroscopy was proposed. The silica-Au/core-shell colloids were synthesized and immobilized on a quartz substrate, and a single-stranded DNA (ssDNA) oligonucleotide designed as an aptamer for coralyne, namely, 5'-SH-(CH2)(6)-AAAAAAAAAAAAAAAA-3', was covalently attached onto the Au nanoshells. The aptamer, with one end on the Au surface, changed conformation upon conjugation with target coralyne, which correspondingly caused changes in the intensity of the Raman band at 1376 cm(-1) and the ratio of the Raman intensity at 736 cm(-1) to the Raman intensity at 1319 cm(-1). The intensity ratio increased with the amount of coralyne added, which therefore allowed the measurement coralyne in a selective and quantitative way, reaching a detection limit as low as 0.1 mu M.
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